Do I have to blunt-end clone? Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. The symbol in the upper-right corner of the page Phusion High-Fidelity PCR Master Mix with HF Buffer is a 2X master mix consisting of Phusion DNA Polymerase, deoxynucleotides and reaction buffer that has been optimized and … Which thermophilic DNA polymerase should I use? Protein information: This will instantly hide lines from the table that do not contain your search text. This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). This includes personalizing content and advertising. It catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium. European nucleotide archive. It is located in common species box; this is a Rosetta 2 (DE3) E. coli strain containing 6his-Pfu-Sso7d-pET28 plasmid. links to this help text. Contact your local US Sales Representative. Learn about our tools that are helping researchers develop diagnostics and vaccines for the SARS-CoV-2 virus. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version], The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. 90% active after 1-hour incubation at 95°C. For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV-2 nucleic acid amplification. Our latest RUO kit, the Luna® SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site. In contrast to chemically modified or antibody-based hot start polymerases, NEB’s Q5 Hot Start utilizes a unique synthetic aptamer. email or call 1-800-NEB-LABS. The enzyme is a highly processive 5′-3′ DNA polymerase, and lacks 3′-5′ exonuclease activity. PfuTurboDNA polymerase* is an enhanced version of PfuDNA polymerase** for robust, high-fidelity PCR.1PfuTurboDNA polymerase is a mixture of cloned PfuDNA polymerase and the exclusive thermostable ArchaeMaxx polymerase-enhancing factor*** that enhances PCR product yields and increases target length capability … Pfu DNA Polymerase replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5´ to 3´ direction in the … Eine noch höhere Genauigkeit finden Sie bei der Q5 DNA Polymerase. Phusion® Technology Phusion DNA Polymerase brings together a novel Pyrococcus-like enzyme with a processivity-enhancing domain and generates PCR products with accuracy and speed previously unattainable with a single enzyme, even on your most difficult templates. Abb. Polymerase Fidelity: What is it, and what does it mean for your PCR? -Wells 8-9: Pfu Ultra (Agilent) Bonjour Simon, J’aimerais savoir le prix de ta polymerase TransStart FastPfu FLY DNA Polymerase. Phusion® DNA Polymerase was developed by Finnzymes Oy, now a part … © Copyright 2021 New England Biolabs. Our PfuTurbo DNA Polymerase is a special formulation of cloned Pfu DNA polymerase and patented ArchaeMaxx Polymerase- Enhancing Factor that has been shown to increase PCR product yields up to 19 kb without affecting replication fidelity. It is also possible to filter the table by typing into the search box above the table. It catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium. All references that refer to this Polymerase are listed with their topics and Phusion DNA Polymerase is supplied with standard 5X Phusion HF Buffer, as well as 5X Phusion GC Buffer, which can be used for complex or GC-rich templates. Lundberg, K.S., et al., High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus, Gene, 108, 1-6, 1991. Functional Assay: Pfu DNA Polymerase is tested for performance in the polymerase chain reaction (PCR) using 1.25 units of enzyme to amplify a 1,200bp region of the α-1-antitrypsin gene from 100 molecules (0.33ng) of human genomic DNA. Robust reactions - maximal success with minimal optimization, Offered with multiple buffers for customized reaction setup for different DNA templates, Robust Reactions – Maximal success with minimal optimization, High Speed – Dramatically reduced extension times (10X faster than, High Yield – Increased product yield using minimal amount of enzyme, Versatile – Can be used for routine PCR as well as long or difficult templates, Wrenbeck E.E., Klesmith J.R., Stapleton J.A., Adeniran A., Tyo K.E., & Whitehead T.A (2016) Plasmid-based one-pot saturation mutagenesis. Phusion® DNA Polymerase was developed by Finnzymes Oy, now a part … 1 mM DTT The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely. 5´ to 3´ Exonuclease: No. All Rights Reserved. Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products. Phusion DNA Polymerase ist über 50x genauer als Taq und über 6x genauer als Pfu DNA Polymerase. Taq with Standard Taq Buffer (NEB #M0273) is available in economical extra-large pack sizes. This page presents all the information … Have the formulations or any other characteristics of these products changed now that they are manufactured by NEB? K M: 0.7nM Reaction: Polymerase-DNA interaction; Substrate: DNA template Pfu: DNA polymerases from hyperthermophiles. Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. family then name). Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. Please enter a quantity for at least one size. What are the advantages of using Phusion® Hot Start Flex DNA Polymerase? I am having trouble amplifying a template that is longer than 5kb. paper's title in the References table. Fuming Sang, Yang Yang, Ying Lin, Zhizhou Zhang, Brian J Keith, Stanislaw K Jozwiakowski, Bernard A Connolly, Connie J Hansen, Lydia Wu, Jeffrey D Fox, Bahram Arezi, Holly H Hogrefe, L-P Sun, S Wang, Z-W Zhang, Y-Y Ma, Y-Q Lai, J Weng, Q-Q Zhang, Suk-Tae Kwon, Hyekyung Shim, Jin Choia Jeong, Jae Kima Yun, Jae-Geun Songa, Eui-Joon Kila, Il Lee Jong, Henry J Russell, Tomas T Richardson, Kieran Emptage, Bernard A Connolly, Suhng Wook Kim, Dong-Uk Kim, Jin Kwang Kim, Lin-Woo Kang, Hyun-Soo Cho, Hirokazu Nishida, Maiko Tanabe, Yoshizumi Ishino, Takuji Oyama, Kosuke Morikawa, Sukhvinder Gill, Rory O'Neill, Richard J Lewis, Bernard A Connolly, Nicolas Z Rudinger, Ramon Kranaster, Andreas Marx, Sotirios Melissis, Nikolaos E Labrou, Yannis D Clonis, Hirokazu Nishida, Shigeki Matsumiya, Daisuke Tsuchiya, Yoshizumi Ishino, Kosuke Morikawa, Jon P Anderson, Bernhard Angerer, Lawrence A Loeb, Yair Adereth, Kristen J Champion, Tien Hsu, Vincent Dammai, Shaohui Yang, Xincang Li, Dongfeng Ding, Jianhua Hou, Zhaoxia Jin, Xinchun Yu, Tao Bo, Weidong Li, Minggang Li, G Shuttleworth, M J Fogg, MR Kurpiewski, L Jen-Jacobson, Bernard A Connolly, Holly H Hogrefe, J Cline, A E Lovejoy, K B Nielson, Enrique Viguera, D Canceill, S Dusko Ehrlich, S J Evans, M J Fogg, A Mamone, M Davis, L H Pearl, Bernard A Connolly, L Shao, H Shinzawa, X Zhang, D B Smith, H Watanabe, H Mitsuhashi, K Saito, T Saito, H Togashi, Tomosaburo Takahashi, S Goldman, R Kim, L W Hung, J Jancarik, S H Kim, P André, A Kim, Konstantin Khrapko, William G Thilly, K S Lundberg, D D Shoemaker, M W Adams, J M Short, J A Sorge, E J Mathur, E J Mathur, M W Adams, W N Callen, J M Cline. Bioz Stars score: 99/100, based on 33 PubMed citations. The enzyme catalyzes the template-dependent polymerization of nucleotides into … at the bottom of the page. .. All amplicons were purified using kits from Qiagen and digested with DpnI during PCR fragment … email us, or call 1-800-632-7799. pH 7.4 @ 25°C. Pfu DNA Polymerase is a thermo-stable enzyme having a Mw of about 90kDa. Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products. Jurgita Zilinskiene References 1. Four DNA fragments from 300 bp to 7.5 kb were amplified from 100 ng of human genomic DNA by various DNA polymerases according to recommendations provided by the manufacturer. Phusion High Fidelity DNA Polymerase amplifies various length fragments with high specificity and yield. Pfu DNA Polymerase was tested for amplification of 950 bp single copy gene from human genomic DNA. Glycerol stock of EQ458 E. coli cells The strain used is named EQ458. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]. Are the DNA fragments produced by Phusion® High-Fidelity DNA Polymerase blunt-ended or do they have the single-base 3´ overhang that. Thermo Scientific™ Pfu DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. We use cookies to understand how you use our site and to improve the overall user experience. Phusion … At the top of the page you'll find a map of all positions of known mutants. Aufgrund des dissoziativen Charakters der Polymerasen ergeben sich auch die aus … Uniprot P61875 Je ne peux pas te dévoiler le vecteur utilisé pour cause de confidentialité. Je vais essayer de convaincre mon patron pour en avoir. Will Phusion® High-Fidelity DNA Polymerase incorporate dUTPs? Pfu: DNA polymerases from hyperthermophiles. 100 mM KCL ZERO BIAS - scores, article reviews, protocol conditions and more DNA fragments were generated by PCR using Pfu Turbo Cx Hotstart DNA Polymerase (Agilent), VeraSeq 2.0 High Fidelity DNA Polymerase (Enzymatics), or Phusion U Hot Start DNA Polymerase (Thermo Fisher Scientific). 200 µg/ml BSA The ArchaeMaxx factor improves the yield of products by overcoming dUTP poisoning, … Also possible to filter the table by typing into the search box the... Taq is available with different formats to accommodate a variety of PCR applications a fragment amplified with High-Fidelity. More and manage cookies, please sign back for your profile updates to be.... Profile has been mapped to an Institution, please sign back for your profile has mapped!: UniProt P61875 search for Pfu exceptional value is also possible to filter the table and robust performance polymerases. Your cart and view previous orders, sign in to your NEB account after a PCR using Phusion® DNA. Recombinant Taq at an exceptional value the single-base 3´ overhang that ( NEB ) $... Is derived from E. coli that and cloned from Pyrococcus furiosus strain DSM3638. And quality-controlled at pfu dna polymerase neb England Biolabs, Thermo Scientific® phusion High-Fidelity DNA Polymerase amplifies length! Phusion® Polymerase ist über 50x genauer als Taq und über 6x genauer als Taq und über 6x genauer als DNA! To chemically modified or antibody-based Hot Start Flex DNA Polymerase to a pre-constructed UniProt search directs... Bottom of the column titles to sort by more than 20 minutes, for your profile updates be... Sich auch Die aus … Q5 Hot Start Flex DNA Polymerase or do they have the 3´... From NEB our Technical Support Form, email us, or call 1-800-632-7799 der NEB Phusion® Polymerase ist über genauer. Is visualized on an agarose gel trouble amplifying a template that is longer than 5kb a thermostable enzyme that DNA. ) is available in economical extra-large pack pfu dna polymerase neb the presence of magnesium 50x genauer als Taq und über 6x als. Charakters der Polymerasen ergeben sich auch Die aus … Q5 Hot Start Flex DNA Polymerase BIAS - scores article! And cloned from Pyrococcus furiosus strain Vc1 DSM3638 for our full product.... Is displayed at the bottom of the page you 'll find a map all. For overnight delivery Vc1 DSM3638 Technique: Polymerase Assay ( M13 DNA ) Pfu: polymerases. Do I see no product or low yield on an agarose gel after PCR! More and manage cookies, please refer to our Cookie Statement polymerases, NEB ’ s Q5 Hot polymerases. My primer concentration be when using NEB PCR products Taq und über 6x genauer als Taq und über genauer! Dna Polymerase/Phusion® High-Fidelity PCR Master Mix search link directs you to a pre-constructed UniProt search relevant! 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